Geographical distribution of Lyme-like borreliosis in Brazil: Hot spots for research and surveillance.

From studies that have accompanied cases of this disease in the country it’s possible to identify that epidemiological, clinical and laboratories aspects are different from cases of Lyme disease recorded in the United States and Eurasia, the Brazilian Lyme borreliosis has been called Lyme-like disease or Syndrome Baggio-Yoshinari.

The etiological agent causer of this disease has not yet been detected (isolation and molecular characterization) in human samples from the cases described in the country and a portion of the medical and scientific community questions the occurrence of the disease in the Brazilian territory.

In order to increase the knowledge about Lyme-like borreliosis in Brazil, we analyzed the records included in the Gerenciador deAmbiente Laboratorial (GAL), which is the information system that collects the data on the requests for laboratory diagnostic tests in the scope of the Brazilian Health System.

Simultaneous identification of Mycoplasma gallisepticum and Mycoplasma synoviae by duplex PCR assay

Mycoplasma gallisepticum (M.G) and Mycoplasma synoviae (M.S) have recognized as common respiratory pathogens especially in chickens cause a lots of economic losses in poultry industries. The aim of this study was development and validation of duplex Polymerase Chain Reaction (PCR) for simultaneous detection of MG and MS. A total of 50 samples from tracheas, lungs and air sacs were taken from commercial broiler chicken farms in Iran. 

The samples were cultured in PPLO broth supplemented for M.S and M.G isolation and bacteria DNA were extracted by phenol/chloroform. The conserved region of 16S rRNA gene was applied for the detection of Mycoplasma genus in 163bp fragment and MG in 183 bp fragment and vlhA gene for detection of MS in 350 bp fragment.