Mycoplasma gallisepticum (M.G) and
Mycoplasma synoviae (M.S) have recognized as common respiratory pathogens
especially in chickens cause a lots of economic losses in poultry industries.
The aim of this study was development and validation of duplex Polymerase Chain Reaction (PCR) for simultaneous detection of MG and MS. A total of 50 samples
from tracheas, lungs and air sacs were taken from commercial broiler chicken
farms in Iran.
The samples were cultured in PPLO broth supplemented for M.S and
M.G isolation and bacteria DNA were extracted by phenol/chloroform. The
conserved region of 16S rRNA gene was applied for the detection of Mycoplasma
genus in 163bp fragment and MG in 183 bp fragment and vlhA gene for detection
of MS in 350 bp fragment.
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