A case of atopic dermatitis that healed completely

The case, which I wish to demonstrate, is that of a 37-year-old man (H.T.), who visited my clinic on September 19, 1991. He had had an atopic dermatitis since the age of 4 years. His dermatitis recovered in part spontaneously at the age of 15 and worsened at the age of 28. He was treated with repeated intra dermal injections with 1 ampoule of Asthremedin, a product of Nippon Pharmaceutical Company(Osaka), consisting of 2 mg peptone and extracts of rabbit’s skin and testis, killed small pox virus, and fungi, at 2-3 day-intervals. Shortly after the 66th injection, he suffered from a temporary worsening of the dermatitis, i.e., a considerable quantity of exudes, followed by a complete healing.

Simultaneous identification of Mycoplasma gallisepticum and Mycoplasma synoviae by duplex PCR assay

Mycoplasma gallisepticum (M.G) and Mycoplasma synoviae (M.S) have recognized as common respiratory pathogens especially in chickens cause a lots of economic losses in poultry industries. The aim of this study was development and validation of duplex Polymerase Chain Reaction (PCR) for simultaneous detection of MG and MS. A total of 50 samples from tracheas, lungs and air sacs were taken from commercial broiler chicken farms in Iran. 

The samples were cultured in PPLO broth supplemented for M.S and M.G isolation and bacteria DNA were extracted by phenol/chloroform. The conserved region of 16S rRNA gene was applied for the detection of Mycoplasma genus in 163bp fragment and MG in 183 bp fragment and vlhA gene for detection of MS in 350 bp fragment.